Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome
If she moves the promoter for the lac operon to the region between the beta galactosidase gene and the permease gene, which of the following would be likely?
A) Three structural genes will no longer be expressed.B) RNA polymerase will no longer transcribe permease.
C) The operon will no longer be inducible.
D) Beta galactosidase will be produced.
E) The cell will continue to metabolize but more slowly.
Answer: D
If she moves the operator to the far end of the operon (past the transacetylase gene), which of the following would likely occur when the cell is exposed to lactose?
A) The inducer will no longer bind to the repressor.
B) The repressor will no longer bind to the operator.C) The operon will never be transcribed.
D) The structural genes will be transcribed continuously.
E) The repressor protein will no longer be produced.
Answer: D
If she moves the repressor gene (lac I), along with its promoter, to a position at some several thousand base pairs away from its normal position, which will you expect to occur?
A) The repressor will no longer be made.B) The repressor will no longer bind to the operator.
C) The repressor will no longer bind to the inducer.
D) The lac operon will be expressed continuously.
E) The lac operon will function normally.
Answer: E
If she moves the operator to a position upstream from the promoter, what would occur?
A) The lac operon will function normally.
B) The lac operon will be expressed continuously.C) The repressor will not be able to bind to the operator.
D) The repressor will bind to the promoter.
E) The repressor will no longer be made.
Answer: B
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